Entries in ohio state (9)
Jeaniene Leis, The Ohio State University
During my trip with HSVMA’s Rural Area Veterinary Services this past summer at the Standing Rock Sioux Reservation in North Dakota, I encountered a unique situation that has left a lasting impact on my life.
Several days in to our week-long clinic, Toshia, a sweet 10-year-old girl from the community, found a very broken 3-month-old kitten under a tree house near her home. She could tell that the tiny kitten was very badly injured and brought her to the community center where our clinic was set up. We examined the kitten and found she would not bear any weight on her swollen left front leg because it was severely fractured. She also had a large wound on her right hind leg that was horribly infested with maggots. Toshia had definitely saved the kitten’s life by bringing her in to the clinic, but her family did not have the ability or resources to care for a kitten in need of such intensive care, and a decision would need to be made about her future.
RAVS is not a rescue group, but a team of veterinary professionals and students passionate about bringing medical care to animals in communities that have limited or no access to veterinary services for their animal family members. Occasionally, like with this kitten, an extreme health or welfare issue puts an animal’s life at stake, especially if there is no family to provide the ongoing care the animal will need.
Sarah Leyman, The Ohio State University
AMPHIBIAN MICROBIOMES AS INDICATORS OF INDIVIDUAL AND ENVIRONMENTAL HEALTH
Sarah Leyman1, Barbara Wolfe1, Paula Mouser2
1The Ohio State University College of Veterinary Medicine, 1900 Coffey Road, Columbus, OH, USA
2The Ohio State University Department of Civil, Environmental and Geodectic Engineering, 2070 Neil Avenue, Columbus, OH, USA
Amphibians depend on their cutaneous microbial community as a first line of immune defense against disease. However, very few studies have been performed to characterize the bacterial genera found on the skin of different amphibian species and under different water quality conditions. The goal of this study was to classify the bacterial genera present on the skin of two Lithobates species living in lakes of highly variant water characteristics on a reclaimed surface mine. A second objective was to develop a baseline frog microbiome library on the site prior to shale gas exploration in order to monitor microbiome changes in association with environmental disturbance. Northern green frogs (Lithobates clamitans melanota) and American bullfrogs (Lithobates catesbeiana) were caught from 10 different lakes on the Wilds in Cumberland, OH. Skin swabs were collected following a sterile saline solution rinse for bacterial characterization and to test for Batrachochytrium dendrobatiditis (Bd), the etiologic agent of amphibian chytridiomycosis. Pharyngeal swabs were taken to test for ranavirus, another emerging disease of amphibians, and blood samples were collected to assess the heterophil-lymphocyte ratio as an indicator of stress. Water quality parameters were documented and water samples collected for chemical analysis at the time of frog capture for each site. The DNA was extracted from the bacterial swabs and sequenced using 454 pyro-sequencing. At least one frog from each site tested was positive for Bd, but no frogs were positive for ranavirus. Water quality among sites varied with regard to pH (4.10 to 8.66), conductivity (137.5 μS/cm to 3.51 mS/cm), ionic content, and dissolved organic carbon (0.13 mg/L to 11.7 mg/L). Our study identified over 300 different genera of microbes representing 68 orders present on frogs on this site. Water quality parameters were found to be associated with differential microbial colonization and physiologic parameters.
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